HEKima™ Adherent HEK Cell SF Media

Scale up your viral vector or gene therapy manufacturing cost-effectively with HEKima Adherent, a QuickStart Media designed to simplify customization and accelerate development and production.
HEKima Adherent HEK cell culture media mobile hero

The HEKima™ Advantage

Fully_Customizable
Customizable
Start with our pre-optimized formula designed for adherent HEK-293 cells and adjust it to fit your process needs in terms of packaging configurations, quality, and overall composition.
Consistent-Quality
Proven Performance
Drive serum-free proliferation in HEK-293, HEK-293T, and HEK-293FT without the need for FBS weaning.
Transparent-Process
Transparent Process
Know every component and concentration and adjust any aspect to match your stage of development, making iteration and scale-up seamless.

Optimize Your HEK Cell Processes With Customizable Adherent HEK Cell Media

Improving production efficiencies is essential to meet the growing demand for lentiviral and AAV vectors which are used in cell and gene therapy manufacturing. HEKima Adherent QuickStart Media provides the opportunity to quickly test and optimize your HEK media enabling you to scale up quickly and cost-effectively.
NB-ROC OH vs OP vs NP graph

Maintains Strong Cell Growth

HEKima Adherent maintains cell proliferation and confluency at equivalent rates after multiple subcultures compared to DMEM + 10% FBS (n=3).

HEKima Adherent HEK cell culture media significantly improves transduction efficiency and protein expression

Significantly Improves Transduction Efficiency and Protein Expression

HEK 293 cells were transduced with a lentivirus expressing GFP. The cells grown in HEKima Adherent showed a higher expression level and more intense signal compared to DMEM + 10% FBS, meaning cells grown in HEKima Adherent lead to an improved protein expression. (n=3)

HEKima Adherent HEK cell culture media showing strong proliferation among the HEK cell variants

Promotes Strong Cell Proliferation for Multiple HEK Variants

HEKima Adherent can support proliferation of different HEK cells variants. When compared to the control media of DMEM + 10% FBS (not shown), the proliferation kinetics for each cell type is comparable.

HEKima Adherent HEK cell culture media significantly improves transduction efficiency and protein expression

Outperforms Competitor Media in HEK Cell Expansion

HEKima Adherent can support HEK cell expansion just as good or better when compared to current industry-leading HEK cell media Panserin 293A or Pro293™.

How QuickStart Media™ Works

As part of our QuickStart Media platform, HEKima Adherent can be ordered as an RUO sample, tested in your process, and then customized and packaged according to your requirements. This service will help streamline the formulation development process of in-demand media products and truly expedite your cell and gene therapy projects.

STEP 1

Order RUO Sample
Complete our request form to order an RUO sample of HEKima Adherent.

STEP 2

Test RUO Sample

Test the performance of HEKima Adherent on your project and review the data.

STEP 3

Customize Your Media
Collaborate with our Sales and Formulation Services teams to tailor the media specifications to your needs.

HEKima™ Adherent HEK Cell Serum-Free Media

Hekima Adherent and coating solution with Physiologix serum replacement

HEKima™ Adherent RUO Sample

Catalog number: 2090
1L

$299

Includes HEKima Adherent Basal Media (1 L), HEKima Adherent Coating Solution (25 mL), and Physiologix Serum Replacement (10 mL).

Hekima Adherent and coating solution

HEKima™ Adherent Custom

Volume: Custom

Inquire for price

Customize HEKima Adherent to meet your requirements. Includes HEKima Adherent Basal Media (1 L) and HEKima Adherent Coating Solution (25 mL).

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Specifications

Specifications below are for the RUO sample. For your custom product, specifications will meet your desired criteria which include the packaging, testing, components, and concentrations.
Type Human Embryonic Kidney 293 (HEK-293) cell medium
Classification Xeno-free, serum-free
Quality Research use
Sterility Sterile filtered basal media
pH 6.8-7.2
Osmolality 345-375 mOsm/Kg
Serum Level Serum-free
Format Liquid
Shipping Conditions HEKima Adherent Medium: 2-8°C
Physiologix XF SR: Dry ice
Storage Temperature HEKima Adherent Basal Media: 2-8°C
HEKima Adherent Coating Solution: 2-8°C
Physiologix XF SR: ≤-70°C
Shelf Life 90 days – analysis ongoing
Research Category HEK-293 cell research, viral production, protein production

Documents and Downloads

Take a closer look at our helpful product information to learn how our cell culture media products and services can support and accelerate your groundbreaking research.

HEKima™ Adherent Product Note

Review the technical details, features, and benefits of HEKima Adherent HEK Cell Serum-free Media.
HEKima™ Adherent Media Performance White Paper
Review HEKima Adherent’s performance with different HEK cell variants compared to DMEM+10% FBS.
Instructions for Use
Review the HEKima Adherent HEK Cell SF Medium instructions for use to ensure proper use.

Need Help?

Get in touch with our customer support team for any questions you may have.

Replace Serum

Supplement your media with Physiologix, a GMP and xeno-free serum replacement.

Formulation Services

Leverage our experts to develop or optimize your media.

FAQs

Have a question? We’re here to help. If you don’t see your question, visit our FAQs page or contact us.
What is HEKima™ Adherent HEK Cell Serum-Free Medium?

HEKima Adherent is a serum-free media developed for use in adherent HEK cells. HEKima Adherent is part of our QuickStart Media portfolio, where it can be supplied as an RUO sample for evaluation, then customized with modifications to components and packaging to meet your desired specifications.

How can I request a sample of HEKima™ Adherent?

Contact our sales team to request a sample of the base formula for HEKima Adherent.

How can I customize and/or order HEKima™ Adherent?

Contact our sales team. After reviewing your project needs, they can coordinate a meeting with our scientific experts to customize and optimize your HEK cell culture media or arrange for shipment of your order.

Why can’t I order or customize HEKima™ Adherent in NB-Lux™?

HEKima Adherent is a proprietary pre-optimized HEK cell culture medium formula. Due to the nature of not being an off-the-shelf product, customers must schedule a meeting with our sales team to order or customize HEKima Adherent HEK Cell Culture Medium.

What cell types has HEKima™ Adherent been tested for?

HEKima Adherent has been tested to perform on different variants of adherent HEK-293 cells. HEKima Adherent was designed to specifically expand adherent HEK cells and boost their protein expression without gradual adaptation to serum-free media. Read our white paper to learn more about HEKima Adherent’s performance.

How much does a customized HEKima™ Adherent medium cost?

Due to the custom nature of HEKima Adherent – customization of components, concentrations, packaging, testing, and quality – pricing is variable. Contact our sales team to request a quote.