In this research spotlight, we feature Dr. Roddy O’Connor at the University of Pennsylvania. Dr. O’Connor works closely with CAR-T luminaries such as Carl June, and now leads his own T cell therapy research team. Download Full Application Note As CAR-T cell therapies...
NB ROC™ T Cell SF Media (cGMP)
OPTIMIZED FOR CAR-T CELL THERAPY
Developed in collaboration with Dr. Roddy O’Connor of the University of Pennsylvania’s Center for Cellular Immunotherapies, NB ROC™ T Cell SF Media boosts transduction efficiency and proliferation while preserving phenotype to support the development of CAR-T cell therapies. In multiple donor T cell studies, NB ROC™ T Cell SF Media used with Physiologix™ XF allowed for a doubling in transduction efficiency, more than 50% higher proliferation and preserved memory-like phenotype when compared to OpTmizer™.
|Manufactured under cGMP using a transparent supply chain|
|Developed in collaboration with Dr. Roddy O’Connor of the University of Pennsylvania’s Center for Cellular Immunotherapies|
|To support transparency in research, each product is supplied with an ingredient list|
|Serum free formula designed for CAR-T cell therapy from development through production|
|Enhanced efficacy when paired with Physiologix™ XF, NB ROC™ is supplied with Physiologix™ XF SR Human Growth Factor Concentrate (hGFC), a cGMP, xeno-free media supplement that has been shown to be superior to human serum in vivo.|
NB ROC™ is configured with the proteins supplied separately for long-term stability and shelf life.
Nucleus Biologics Develops cGMP CMRL Media and zIF™ Xeno-Free Supplement for Robust Human Islet Cell Culture
SAN DIEGO – February 18, 2020 – Nucleus Biologics™, a leader in precision cell culture, announced today a complete line of cGMP grade media and a new supplement to enable improved insulin response and standardized processing of human pancreatic islets and beta cells....
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Using a combination of metabolomics and process optimization, Nucleus Biologics has identified a new xeno- and serum-free cell culture medium formulation named NB ROC. In this study, we tested NB ROC on T cells isolated from the blood of three donors using standard protocols. Our data show that NB ROC allowed cells to grow 50% more compared to OpTmizer (Fisher Scientific). This increase in proliferation did not compromise early memory phenotypes. Additionally, transduction using a GFP lentivirus was ~40% higher when cells were grown and activated in NB ROC, which results in optimization of virus usage and reduction of manufacturing costs.