NB-ROC™ T Cell SF Media
Speed your time to the clinic and create a more potent CAR T-cell therapy with NB-ROC, a xeno-free, serum-free T cell culture media that can significantly boost transduction efficiency.
The NB-ROC™ Advantage
Boost Transduction Efficiency
Lower Manufacturing Cost
Decrease the amount of viral vector used in your CAR-T process, which can result in reduced cost and increased safety of the final therapeutic dose.
Maintain Key Quality Indicators
Maximize T Cell Performance
The right media can ease development challenges and impact speed, cost, and quality of the therapeutic dose. NB-ROC is optimized for CAR-T cell therapy development, primarily focused on significantly improving transduction, while maintaining all other critical attributes.
Significantly Improves Transduction Efficiency
Combining NB-ROC with 2% Physiologix (NP), a serum-free complete media, improves transduction efficiency when compared to OpTmizer™ + 5% Human Serum (OH) and OpTmizer + 2% Physiologix (OP). Data shown represents average of 3 donors ±SD in a G-Rex® model.
Promotes Strong T Cell Growth
NB-ROC + 2% Physiologix (NP) supports strong population doubling equivalent to OpTmizer™ + 5% Human Serum (OH) and OpTmizer + 2% Physiologix (OP). Proliferation, cell health, viability, and maintenance of relevant phenotypes (assessed but not shown) are also comparable. Data shown represents average of 3 donors ±SD in a G-Rex® model.
Increases CAR+ Cells in Your Therapeutic Dose
NB-ROC increases efficacious cells in your process. Using GFP as a marker of lentiviral transduction, NB-ROC + 2% Physiologix (NP) shows significantly higher numbers of transfected cells compared to OpTmizer™ + 5% Human Serum (OH) and OpTmizer + 2% Physiologix (OP) after 7 days of culture. Data shown represents average of 3 donors ±SD in a G-Rex® model.
NB-ROC™ T Cell Serum-Free Media
NB-ROC™
Catalog number: 1536
1 L
$209
Includes NB-ROC T Cell Basal Media (1 L) and NB-ROC T Cell Supplement (25 mL). NB-ROC is configured with the proteins supplied separately for long-term stability and shelf life.
NB-ROC™ with Physiologix™ Kit
Catalog number: 1537
1 L
$366
Kit includes NB-ROC T Cell Basal Media (1 L), NB-ROC T Cell Supplement (25 mL), and Physiologix (2 x 10 mL). NB-ROC is configured with the proteins supplied separately for long-term stability and shelf life.
NB-ROC™ GMP
Catalog number: Coming soon
1 L
Inquire for price
Includes NB-ROC T Cell Basal Media (1 L) and NB-ROC T Cell Supplement (25 mL). NB-ROC is configured with the proteins supplied separately for long-term stability and shelf life.
NB-ROC™ GMP with Physiologix™ Kit
Catalog number: Coming soon
1 L
Inquire for price
Kit includes NB-ROC T Cell Basal Media (1 L), NB-ROC T Cell Supplement (25 mL), and Physiologix (2 x 10 mL). NB-ROC is configured with the proteins supplied separately for long-term stability and shelf life.
Specifications
| Type | T cell medium |
| Classification | Xeno-free, serum-free |
| Quality | Research use |
| Sterility | Sterile filter basal media |
| pH | 6.5 to 7.5 |
| Osmolality | 270-330 mOsm/Kg |
| Included Antibiotics | No antibiotics |
| Phenol Red Indicator | Phenol red free |
| Serum Level | Serum-free |
| Format | Liquid |
| Glutamine | Yes |
| Sodium Pyruvate Additive | Yes |
| Shipping Conditions | 2-8°C |
| Storage Temperature | NB-ROC T cell basal media: 2-8°C NB-ROC T cell supplement: 2-8°C Physiologix: ≤-70°C |
| Shelf Life |
365 days if not mixed Recommended to use media within 2 weeks of mixing the basal media and supplement |
| Research Category | T cell research |
Documents and Downloads
Take a closer look at our helpful product information to learn how our cell culture media products and services can support and accelerate your groundbreaking research.
NB-ROC™ Product Note
Sample Certificate of Analysis
Review a sample of our standard certificate of analysis (COA).
Instructions for Use
Review the NB-ROC instructions for use to ensure proper use.
Need Help?
Get in touch with our customer support team for any questions you may have.
Visit NB-Lux™
Optimize Performance
Supplement your media with Physiologix, a GMP and xeno-free serum replacement.
Resources
Explore our educational resources to learn how NB-ROC can be a competitive advantage on your path to commercialization.
FAQs
What is NB-ROC™ T Cell Serum-Free Medium?
NB-ROC is a T cell serum-free media that significantly boosts transduction efficiency while maintaining proliferation and preserving relevant phenotypes to support the development of CAR-T cell therapies.
Have you tested NB-ROC™ with other cell types?
Does NB-ROC™ only work when supplemented with Physiologix™?
NB-ROC can be supplemented with a variety of proteins including human serum. To achieve up to threefold improvement in transduction efficiency, however, Physiologix must be used.
Physiologix is a serum replacement created by Nucleus Biologics that is recommended for its xeno-free and serum-free properties, as well as its enhanced synergy with NB-ROC. To achieve the highest transduction efficiency possible, consider supplementing with Physiologix.
Has NB-ROC™ been tested with other activation materials?
NB-ROC has only been tested with Dynabead activation.
Can I customize NB-ROC™?
NB-ROC can be customized through formulation services and licensing.