NB-ROC™ T Cell Serum-Free Media

OPTIMIZED FOR CAR-T CELL THERAPY DEVELOPMENT

Developed in collaboration with T cell researchers at the University of Pennsylvania’s Center for Cellular Immunotherapies, NB-ROC T Cell SF Media significantly boosts transduction efficiency while maintaining proliferation and preserving relevant phenotypes to support the development of CAR-T cell therapies. In multiple donor T cell studies, NB-ROC T Cell SF Media used with Physiologix™ improved transduction efficiency by threefold when compared to a leading T-cell media and human serum.

Manufactured in an ISO 13485-certified facility – ensuring lot-to-lot consistency. GMP formula will be available in early 2023.

Significantly improves transduction efficiency, maintains desired phenotypes, and promotes proliferation and population doubling.

Developed in collaboration with T cell researchers to deliver a more potent and cost-effective therapy.

Manufactured in lot sizes of up to 2,000 liters and in a facility with a capacity of millions of liters per year.

Serum-free formula designed for use in clinical development and scale up.

Enhanced transduction efficiency when paired with Physiologix.

Superior Transduction Efficiency

Benefits of Increased Transduction Efficiency

  • Lower MOI allows less virus use
  • More CAR-positive cells
  • Reduced cost
  • Increased safety of final therapeutic dose

Comparison of T cell Lentivirus transduction in different T cell culture media: an industry-leading T cell media (TCM) + 5% Human Serum (green), TCM + 2% Physiologix (blue), and NB-ROC + 2% Physiologix (yellow).​

Cell culture started at D0 using a cell density of 0.5 x 106 cells/mL in a G-REX 10 vessel (6 well, 40 mL volume media/well). Lentivirus expressing GFP (Green Fluorescent Protein) was added on D1. On D5, 30 mL of media was removed, cells were resuspended, and GFP+ expression using Flow Cytometry was assessed.

Promotes Strong T Cell Proliferation

Promotes proliferation and doubling as good as or better than current off-the-shelf T-cell media options. All other quality indicators, like cell health and viability, are also maintained.

Comparison of T cell proliferation and population doubling in different T cell culture media: an industry-leading T cell media (TCM) + 5% Human Serum, TCM + 2% Physiologix and, NB-ROC + 2% Physiologix.​

Cell culture started at D0 using a cell density of 0.5 x 106 cells/mL in a G-REX 10 vessel (6 well, 40 mL volume media/well). On D5, 30 mL of media was removed, cells were resuspended, and cell count was performed using a cell counter (DeNovix CellDrop Cell Counter), and 30 mL of fresh media was added. Same steps were followed for day 5 and Day 7. Population doubling was calculated using the final cell count.​

Ordering Information

NB-ROC is configured with the proteins supplied separately for long-term stability and shelf life.

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