NB-ROC™ T Cell Serum-Free Media
OPTIMIZED FOR CAR-T CELL THERAPY DEVELOPMENT
Developed in collaboration with T cell researchers at the University of Pennsylvania’s Center for Cellular Immunotherapies, NB-ROC T Cell SF Media significantly boosts transduction efficiency while maintaining proliferation and preserving relevant phenotypes to support the development of CAR-T cell therapies. In multiple donor T cell studies, NB-ROC T Cell SF Media used with Physiologix™ improved transduction efficiency by threefold when compared to a leading T-cell media and human serum.
Manufactured in an ISO 13485-certified facility – ensuring lot-to-lot consistency. GMP formula will be available in early 2023.
Significantly improves transduction efficiency, maintains desired phenotypes, and promotes proliferation and population doubling.
Developed in collaboration with T cell researchers to deliver a more potent and cost-effective therapy.
Serum-free formula designed for use in clinical development and scale up.
Superior Transduction Efficiency
Benefits of Increased Transduction Efficiency
- Lower MOI allows less virus use
- More CAR-positive cells
- Reduced cost
- Increased safety of final therapeutic dose
Comparison of T cell Lentivirus transduction in different T cell culture media: an industry-leading T cell media (TCM) + 5% Human Serum (green), TCM + 2% Physiologix (blue), and NB-ROC + 2% Physiologix (yellow).
Cell culture started at D0 using a cell density of 0.5 x 106 cells/mL in a G-REX 10 vessel (6 well, 40 mL volume media/well). Lentivirus expressing GFP (Green Fluorescent Protein) was added on D1. On D5, 30 mL of media was removed, cells were resuspended, and GFP+ expression using Flow Cytometry was assessed.
Promotes Strong T Cell Proliferation
Promotes proliferation and doubling as good as or better than current off-the-shelf T-cell media options. All other quality indicators, like cell health and viability, are also maintained.
Comparison of T cell proliferation and population doubling in different T cell culture media: an industry-leading T cell media (TCM) + 5% Human Serum, TCM + 2% Physiologix and, NB-ROC + 2% Physiologix.
Cell culture started at D0 using a cell density of 0.5 x 106 cells/mL in a G-REX 10 vessel (6 well, 40 mL volume media/well). On D5, 30 mL of media was removed, cells were resuspended, and cell count was performed using a cell counter (DeNovix CellDrop Cell Counter), and 30 mL of fresh media was added. Same steps were followed for day 5 and Day 7. Population doubling was calculated using the final cell count.
NB-ROC is configured with the proteins supplied separately for long-term stability and shelf life.
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