NB ROC™ T Cell SF Media (cGMP)


Developed in collaboration with Dr. Roddy O’Connor of the University of Pennsylvania’s Center for Cellular Immunotherapies, NB ROC™ T Cell SF Media boosts transduction efficiency and proliferation while preserving phenotype to support the development of CAR-T cell therapies. In multiple donor T cell studies, NB ROC™ T Cell SF Media used with Physiologix™ XF allowed for a doubling in transduction efficiency, more than 50% higher proliferation and preserved memory-like phenotype when compared to OpTmizer™.

Manufactured under cGMP using a transparent supply chain
  • Increased transduction efficiency by 40%
  • >50% increase in proliferation
  • Preservation of memory-like phenotype
Developed in collaboration with Dr. Roddy O’Connor of the University of Pennsylvania’s Center for Cellular Immunotherapies
To support transparency in research, each product is supplied with an ingredient list
Serum free formula designed for CAR-T cell therapy from development through production
Enhanced efficacy when paired with Physiologix™ XF, NB ROC™ is supplied with Physiologix™ XF SR Human Growth Factor Concentrate (hGFC), a cGMP, xeno-free media supplement that has been shown to be superior to human serum in vivo.

Ordering Information

NB ROC™ is configured with the proteins supplied separately for long-term stability and shelf life. 

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NB ROC T Cell Poster

Authors: Asma Ayari, Francisco J Martinez-Becerra, Yinan Tu, Yinan Lu, and Roddy O’Connor 

ROC T cells

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Using a combination of metabolomics and process optimization, Nucleus Biologics has identified a new xeno- and serum-free cell culture medium formulation named NB ROC. In this study, we tested NB ROC on T cells isolated from the blood of three donors using standard protocols. Our data show that NB ROC allowed cells to grow 50% more compared to OpTmizer (Fisher Scientific). This increase in proliferation did not compromise early memory phenotypes. Additionally, transduction using a GFP lentivirus was ~40% higher when cells were grown and activated in NB ROC, which results in optimization of virus usage and reduction of manufacturing costs.