Video Library

Increase T Cell Proliferation

In this segment, we talk about how CD4+ T cells grown in Physiologix™ XF and RPMI 1640 performs versus an industry leading off-the-shelf serum free media. The cells grown in the Physiologix XF show higher viable cell counts compared to SFM. Watch the video for full details.

T Cell Surface Marker Expression

In this segment, we talk about surface marker expression of CD4+ T cells activated with anti-hCD3 / CD28 / CD2 for 7 days. Cells grown in Physiologix showed higher capability for activation and exhibited greater ability for exhaustion which is indicative of a more physiologically relevant response to constant 7 day stress compared to the […]

Functional Consistency in MSC Serum Replacement

In this segment, we show data of bone marrow derived mesenchymal stem cells being grown in five different lots of Physiologix. As you can see from the data we confirmed that Physiologix has great functional consistency across multiple lots. Lack of consistency often wastes valuable time and resources. Using Physiologix XF Serum Replacement could equate […]

Increase iPSC Proliferation

In this segment, we show data of commercially available induced pluripotent stem cells (iPSCs) being grown in an industry leading off-the-shelf serum-free media alongside with Physiologix at 2% concentration in DMEM/F12. The data shows, over eight days, the cells grown in the Physiologix supplemented media show higher viable cell counts compared to SFM. This data […]

Maintaining Phenotypic Fidelity of iPSCs After Passaging

We shared proliferation data in a previous video showing enhanced cell growth using Physiologix in iPSCs. While growth is important, it is equally important to determine if the iPSCs maintained phenotypic fidelity after passaging. In this experiment, we determined the pre-passaging levels of iPSC surface markers CD44, Oct4, Sox2 and SSEA-4 using flow cytometry. We […]