Classical Media per Your Requirements

Our classical media is made exactly following your specifications. Don’t search catalogs of part numbers and settle on something close. Add or modify any component and receive media ready to pipette.

Classical Cell Culture Media

Premium, Consistent, and Flexible Classical Media

Customizable Classical Media

Configure any aspect of your selected media to fit your needs – from components and concentration to testing, grade, and packaging configurations.

GMP Manufacturing

Media is manufactured under GMP conditions and governed by strict adherence to an ISO 13485:2016-certified quality system. 

Simplified Ordering

Easily request a quote or place and track your order through through NB-Lux. Customizing and ordering through NB-Lux gives you full transparency of components and concentrations, and expedites the ordering process.

Classical Media Customized to Your Specifications in 3 Easy Steps

Classical media serve as an essential base for the design of formulations to support cell growth. With our unique approach, infinitely customize your classical media to meet your exact specifications.


Choose an Existing Classical Media

Select from the media options listed below and click “order now” to access NB-Lux to configure and order.


View and Customize Your Media

Customize every aspect of your media – packaging, grade, testing, components, and component concentrations.


Place Your Order

Confirm each section of the customization process – Packaging, Testing, and Formulation – then save to cart, request a quote, or place an order.

Explore Classical Cell Culture Media

CMRL 1066 Medium (Basal)
CMRL 1066 Medium (Basal)
CMRL 1066 Medium (Supplemented)
CMRL 1066 Medium (Supplemented)
CMRL 1066 Medium (CIT Modification)
CMRL 1066 Medium (CIT Modification)
Dulbecco's Modified Eagle Medium (DMEM)
Dulbecco's Modified Eagle Medium (DMEM)
Eagle's Minimum Essential Medium (EMEM)
Eagle's Minimum Essential Medium (EMEM)
Ham’s F-10 Nutrient Mix
Ham’s F-10 Nutrient Mix
Ham’s F-12 Nutrient Mix
Ham’s F-12 Nutrient Mix
Iscove's Modified Dulbecco's Medium (IMDM)
Iscove's Modified Dulbecco's Medium (IMDM)
McCoy’s 5A Medium
McCoy’s 5A Medium
Medium 199
Medium 199
Minimum Essential Medium (MEM)
Minimum Essential Medium (MEM)
RPMI 1640 Medium
RPMI 1640 Medium
Manufacture at your bench

Manufacture Classical Media at Point-Of-Use With Krakatoa™

Order powdered media in recyclable pods. Krakatoa presents a simple solution to easily deliver fresh point-of-use cell media right in your laboratory. Reduce greenhouse emissions by 65% and significantly reduce the use of single-use plastics in cell culture.

Documents and Downloads

Take a closer look at our helpful product information to learn how our cell culture media products and services can support and accelerate your groundbreaking research.

NB-Lux™ Product Note

Learn more about NB-Lux and the benefits of customizing your media via this online media configurator.

Krakatoa™ Product Note

Review the technical details, features, and benefits of the Krakatoa media maker.

Need Help?

Get in touch with our customer support team for any questions you may have.

Formulation Services

Get expert help in developing or optimizing your media.

Optimize Performance

Supplement your media with Physiologix, a GMP and xeno-free serum replacement.


Have a question? We’re here to help. If you don’t see your question, visit our FAQs page or contact us.
What is classical cell culture media?

Cell culture medium is a liquid or gel designed to support the growth and maintenance of a variety of cells and cell lines. Classical media are the first generation of cell culture media and have been used in labs for decades. Media contains inorganic salts, amino acids, vitamins, and other components needed for optimal cell growth.

Can I customize classical cell culture media?

Yes. When clicking “Order now,” you will be navigated to NB-Lux, our online ordering and customization tool. In NB-Lux, you can add or remove components, adjust concentrations, select sizing and testing options, modify packaging configurations, and order your media.

Additional services like NB-AIR and Formulation Services offer media design and customization assistance.

If you prefer to work directly with one of our Technical Sales Specialist, contact us and a specialist will reach out to you to discuss your project.

Are there resources to learn how to design and customize media using NB-AIR™ and NB-Lux™?

We have numerous webinars and videos available on our website on how to design and customize media using our easy-to-use cloud-based tools. If you would like to schedule a personal walkthrough of NB-AIR and/or NB-Lux, contact us to schedule a demo with one of our Technical Sales Specialists.

Visit our resources page to access our educational tools to learn more about our tools.

Can I order classical media without customization?

Yes. For each classical media we offer, we have a recommended formula. Simply select your packaging configuration, quality, and required testing prior to placing an order or requesting a quote.

Can I order complete liquid media?

Yes. Select from a list of common proteins or add our serum replacement, Physiologix XF SR, for a ready-to-pipette complete media solution.

In NB-Lux™, it says you have the largest library of over 400 publicly available formulas to choose from. What does that mean?

Basal media formulations exist in the public domain for anyone to use. In NB-Lux, users have the ability to start from a specific supplier’s formula simply by inputting their catalog number, keyword, or cell type.

Additionally, Nucleus Biologics has specific recommended formulations we use for our classical media.