Vitronectin XF is a recombinant, extracellular matrix (ECM) that promotes cell proliferation, maintains pluripotency, and supports normal colony morphology for MSCs and iPSCs.
The Vitronectin XF™ Advantage
Comprised of a single, defined, recombinant full-length human vitronectin sequence expressed under chemically-defined conditions.
Prepared under xeno-free conditions, create a completely xeno-free system when using Vitronectin XF with chemically-defined media.
Easy to Use and Proven
Can be used at room temperature and has demonstrated ability to support normal colony morphology, proliferation, and pluripotency in iPSCs and MSCs.
Standardize Stem Cell Process Development
Take control over your culture environment with Vitronectin XF and gain more consistent cell populations and reproducible results in downstream applications.
Supports Cell Stemness and Viability in MSCs
Vitronectin XF performs equivalently to Matrigel® ECM in attributes critical to the success of MSC studies. Pluripotency as assessed by the expression of CD90, cellular morphology, as well as proliferation and viability (data not shown) are equivalent to the murine-based Matrigel ECM. As a defined, xeno-free ECM, Vitronectin XF is a superior choice to streamline regulatory hurdles and consistency in your development.
Maintains Pluripotency in iPSCs
Cryopreserved human induced pluripotent stem cells (hiPSCs) were thawed and recovered, then split on Vitronectin XF plates. After 5 passages, the cells were subjected to flow cytometry testing for the pluripotency markers Oct4 (yellow curve), SSEA4 (not shown), and Tra1-81 (not shown). The green curve indicates negative control cells.
Maintains Normal Colony Morphology in iPSCs
Morphological characteristics play a critical role in indicating pluripotency in iPSCs, and in determining the time of passaging in both iPSC and MSC assays. Vitronectin XF maintains normal colony morphology for iPSCs comparably to Matrigel®.
Vitronectin XF™ Extracellular Matrix
Catalog number: 520
|Type||Recombinant human ECM|
|Purity||≥ 85% by SDS-PAGE and Coomassie blue staining|
|Sterility||No growth at 14 days|
|Endotoxin||≤ 1.25 EU/mL|
|Shipping Conditions||Dry ice|
|Shelf Life||365 days|
|Research Category||Stem cell research|
Documents and Downloads
Take a closer look at our helpful product information to learn how our cell culture media products and services can support and accelerate your groundbreaking research.
Vitronectin XF™ Product Note
Review the technical details, features, and benefits of Vitronectin XF extracellular matrix.
Sample Certificate of Analysis
Review a sample of our standard certificate of analysis (COA).
Instructions for Use
Review the Vitronectin XF instructions for use to ensure proper use.
Get in touch with our customer support team for any questions you may have.
View and order all available supplements and cell culture media products.
Need Cell Media?
Explore our portfolio of classical, specialized, and custom cell culture media.
Explore our educational resources to learn how Vitronectin XF can be a competitive advantage to your program.
What is Vitronectin XF™?
Vitronectin XF is a recombinant, extracellular matrix (ECM) that promotes cell proliferation, maintains pluripotency, and supports normal colony morphology for mesenchymal (MSC) and induced pluripotent stem cells (iPSCs). Vitronectin XF is xeno-free and supports cell growth and differentiation under serum-free, feeder-free conditions.
Is Vitronectin XF™ chemically defined?
Vitronectin XF is comprised of a single, defined, recombinant full-length human vitronectin sequence expressed under chemically-defined conditions.
What is the difference between Vitronectin XF™ and Corning Matrigel®?
Both products are extracellular matrices that support morphology, promote proliferation, and maintain pluripotency. However, key features such as the ability to use non-treated well plates, storing and using Vitronectin XF at room temperature, or the fact that Vitronectin XF is xeno-free and defined makes Vitronectin XF a great, easy-to-use choice that can simplify research and streamline regulatory compliance as you scale.
Learn more about the differences by viewing our infographic.
Can the working solution be used multiple times?
Can plates coated with Vitronectin XF™ be stored at room temperature?
We recommend that plates coated with Vitronectin XF are stored at 2-8°C.