Vitronectin XF™
Introducing

Vitronectin XF™

Xeno-Free Extracellular Matrix

Formerly by Primorigen Biosciences, now acquired by Nucleus Biologics. A recombinant extracellular matrix (ECM) that promotes cell proliferation, maintains pluripotency and supports normal colony morphology for mesenchymal and induced pluripotent stem cells.

$55 / 2 mL (500 µg @ 250 µg/mL)
Part Number: 10230
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Testing:

Mycoplasma Sterility
Endotoxin Protein Purity

Sample CoA

(Click to download) Vitronectin XF™ - Sample Certificate of Analysis

Instructions for Use

(Click to download) Vitronectin XF™ - Instructions for Use

Maintains Pluripotency

Vitronectin XF™ - Pluripotency

Maintains Normal Colony Morphology

Vitronectin XF™ - Colony Morphology

Differentiation to Definitive Endoderm

Human induced pluripotent stem cells (hiPSCs) were grown on Vitronectin XF™ plates. After 5 passages the cells were replated on Vitronectin XF™ and subjected to Definitive Endoderm (DE) differentiation using a protocol adapted from Si-Tayeb et al, 2010, Hepatology 51:297. After the initial 5 day exposure to Activin A/BMP-4/FGFb, the cells were harvested and analyzed by flow cytometry for the DE markers GATA4, CXCR4, and FoxA2, and for the absence of the pluripotency marker Oct4.

Vitronectin XF™ - Definitive Endoderm

Hepatocyte Differentiation

Human pluripotent stem cells (hESCs and hiPSCs) were plated on Vitronectin XF™ and subjected to hepatocyte differentiation using a protocol adapted from Si-Tayeb et al, 2010, Hepatology 51:297. After the initial 5 day exposure to Activin A/BMP-4/FGFb, the cells were stained for presence of GATA4 and absence of Oct4. Data courtesy of M. Nagaoka and S. Duncan, Medical College of Wisconsin.

Vitronectin XF™ - Hepatocyte Differentiation 1

Cells were fixed and stained at Days 10, 15, and 20 of the hepatocyte differentiation protocol. Cells expressing hepatocyte markers HNF4A (red), alpha-fetoprotein (AFP; green), and Albumin (ALB; green) were detected by immunocytochemistry. Nuclei were counterstained with DAPI. These data suggest mature adult-like hepatocytes can be generated on Primorigen’s Vitronectin XF™. Data courtesy of M. Nagaoka and S. Duncan, Medical College of Wisconsin.

Vitronectin XF™ - Hepatocyte Differentiation 2