Vitronectin XF™

Vitronectin XF is a recombinant, extracellular matrix (ECM) that promotes cell proliferation, maintains pluripotency, and supports normal colony morphology for MSCs and iPSCs.

Vitronectin XF

The Vitronectin XF™ Advantage

Defined
Defined

Comprised of a single, defined, recombinant full-length human vitronectin sequence expressed under chemically-defined conditions.

Xeno-Free
Xeno-Free

Prepared under xeno-free conditions, create a completely xeno-free system when using Vitronectin XF with chemically-defined media.

Easy_To_Use_And_Proven
Easy to Use and Proven

Can be used at room temperature and has demonstrated ability to support normal colony morphology, proliferation, and pluripotency in iPSCs and MSCs.

Standardize Stem Cell Process Development

Take control over your culture environment with Vitronectin XF and gain more consistent cell populations and reproducible results in downstream applications.

Supports-cell-stemness-and-viability-in-MSCs_1

Supports Cell Stemness and Viability in MSCs

Vitronectin XF performs equivalently to Matrigel® ECM in attributes critical to the success of MSC studies. Pluripotency as assessed by the expression of CD90, cellular morphology, as well as proliferation and viability (data not shown) are equivalent to the murine-based Matrigel ECM. As a defined, xeno-free ECM, Vitronectin XF is a superior choice to streamline regulatory hurdles and consistency in your development.

Mainrains Pluripotency in IPSCs

Maintains Pluripotency in iPSCs

Cryopreserved human induced pluripotent stem cells (hiPSCs) were thawed and recovered, then split on Vitronectin XF plates. After 5 passages, the cells were subjected to flow cytometry testing for the pluripotency markers Oct4 (yellow curve), SSEA4 (not shown), and Tra1-81 (not shown). The green curve indicates negative control cells.

Matrigel vs Vitronectin

Maintains Normal Colony Morphology in iPSCs

Morphological characteristics play a critical role in indicating pluripotency in iPSCs, and in determining the time of passaging in both iPSC and MSC assays. Vitronectin XF maintains normal colony morphology for iPSCs comparably to Matrigel®.

Vitronectin XF™ Extracellular Matrix

Vitronectin XF-Current

Vitronectin XF™

Catalog number: 520
2 mL

$85.00

Specifications

Type Recombinant human ECM
Classification Xeno-free
Concentration 250 µg/mL
Volume 2 mL
Purity ≥ 85% by SDS-PAGE and Coomassie blue staining
Sterility No growth at 14 days
Endotoxin ≤ 1.25 EU/mL
Mycoplasma Negative
Shipping Conditions Dry ice
Storage Temperature -80°C
Shelf Life 365 days
Research Category Stem cell research

Documents and Downloads

Take a closer look at our helpful product information to learn how our cell culture media products and services can support and accelerate your groundbreaking research.

Vitronectin XF™ Product Note

Review the technical details, features, and benefits of Vitronectin XF extracellular matrix.

Sample Certificate of Analysis

Review a sample of our standard certificate of analysis (COA).

Instructions for Use

Review the Vitronectin XF instructions for use to ensure proper use.

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FAQs

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What is Vitronectin XF™?

Vitronectin XF is a recombinant, extracellular matrix (ECM) that promotes cell proliferation, maintains pluripotency, and supports normal colony morphology for mesenchymal (MSC) and induced pluripotent stem cells (iPSCs). Vitronectin XF is xeno-free and supports cell growth and differentiation under serum-free, feeder-free conditions.

Is Vitronectin XF™ chemically defined?

Vitronectin XF is comprised of a single, defined, recombinant full-length human vitronectin sequence expressed under chemically-defined conditions.

What is the difference between Vitronectin XF™ and Corning Matrigel®?

Both products are extracellular matrices that support morphology, promote proliferation, and maintain pluripotency. However, key features such as the ability to use non-treated well plates, storing and using Vitronectin XF at room temperature, or the fact that Vitronectin XF is xeno-free and defined makes Vitronectin XF a great, easy-to-use choice that can simplify research and streamline regulatory compliance as you scale.

Learn more about the differences by viewing our infographic.

Can the working solution be used multiple times?
We recommend using the working solution up to 2 times.
Can plates coated with Vitronectin XF™ be stored at room temperature?

We recommend that plates coated with Vitronectin XF are stored at 2-8°C.