NB-MSC™ SF Media

NB-MSC is a high-performing, cost-effective, serum- and xeno-free QuickStart MSC culture media that is ready to use or customizable to meet your process needs. Configure components, concentrations, packaging, and quality.

Nucleus Biologics' NB-MSC cell culture medium in a laboratory.

The NB-MSC™ Advantage

Fully_Customizable
Customizable

Start with our pre-optimized MSC media formula or use it as a base to adjust to your process needs in terms of packaging configurations, quality, and overall composition.

Consistent-Quality
Cost-Effective Performance

Drive serum-free proliferation at superior levels to the gold standard DMEM + 10% FBS while realizing cost savings versus other off-the-shelf media.

Transparent-Process
Transparent Process

Know every component and concentration and adjust any aspect to match your stage of development, making iteration and scale-up seamless.

Maximize Performance With Xeno-Free MSC Media

MSCs are key players in cell therapy and regenerative medicine due to their significant therapeutic potential. However, traditional MSC culture systems often rely on FBS supplementation, which introduces variability and risks associated with animal-derived components. NB-MSC medium is serum-free, xeno-free, and pre-optimized to enhance MSC proliferation, offering a more consistent and reliable alternative to FBS-based media, which is critical to address regulatory concerns during downstream development. As part of our QuickStart Media platform, NB-MSC is fully customizable, providing added convenience and cost-efficiency, along with the flexibility to meet specific research and production needs.

Enhanced MSC proliferation without FBS when using NB-MSC medium.

Enhanced MSC Proliferation Without FBS

Cell counts following green fluorescent dye staining revealed that MSCs cultured in NB-MSC media supplemented with 1% Physiologix shows a significantly higher number of cells per well than either DMEM with 10% FBS or DMEM with 5% FBS at day 14. This data demonstrates NB-MSC’s ability to support superior MSC proliferation, offering a more effective alternative to FBS-containing media.

Earlier and faster MSC confluency when using NB-MSC.

Earlier and Faster Confluency with NB-MSC™

NB-MSC media significantly accelerates MSC proliferation, as shown by confluency measurements over time. When supplemented with 1% Physiologix, NB-MSC achieves 100% confluency by day 6, highlighting its rapid support for MSC growth. In contrast, DMEM with 10% FBS reached only 80% confluency by day 6, while DMEM with 5% FBS attained just 40% confluency at the same time point. This data emphasizes NB-MSC’s ability to facilitate faster and more complete cell coverage compared to FBS-containing alternatives.
CD90 expression comparison - NB-MSC vs DMEM+10% FBS

Maintains CD90 Expression

CD90 expression was assessed on day 7 following expansion in both NB-MSC and DMEM + 10% FBS culture conditions. The results demonstrate comparable levels of expression across the two media, indicating that culturing cells in NB-MSC preserves their undifferentiated state while effectively supporting their expansion.

How QuickStart Media™ Works

As part of our QuickStart Media platform, NB-MSC can be ordered as an RUO sample, tested in your process, and then customized and packaged according to your requirements. This service will help streamline the formulation development process of in-demand media products and expedite your cell therapy and regenerative medicine projects.

STEP 1

Order RUO Sample

Complete our request form to order an RUO sample of NB-MSC.

STEP 2

Test RUO Sample

Test the performance of NB-MSC on your project and review the data.

STEP 3

Customize Your Media
Collaborate with our Sales and Formulation Services teams to tailor the media specifications to your needs.

NB-MSC™ Serum-Free Media

NB-MSC basal media. coating solution, and Physiologix serum replacement.

NB-MSC™ RUO Sample

Catalog number: 2127
1 L

$549

Includes NB-MSC Basal Medium (1 L), NB-MSC Coating Solution (25 mL), and Physiologix Serum Replacement (10 mL).

NB-MSC basal medium and coating solution.

NB-MSC™ Custom

Volume: Custom

Inquire for price

Customize NB-MSC to meet your requirements. Includes NB-MSC Basal Medium (1 L) and NB-MSC Coating Solution (25 mL).

Consent
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Specifications

Specifications below are for the RUO sample. For your custom product, specifications will meet your critical quality attributes which include the packaging, testing, components, and concentrations that you desire.
Type Mesenchymal stem cell (MSC) medium
Classification Xeno-free, serum-free
Quality Research use (RUO)
Sterility Sterile filtered through a 0.2 micron filter
Format Liquid
Volume 1 L
pH 6.8 to 7.2
Osmolality 345-375 mOsm/Kg
Phenol Red Indicator Yes (can be removed)
Shipping Conditions NB-MSC Medium: 2-8°C
Physiologix XF SR: Dry ice
Storage Temperature NB-MSC Basal Media: 2-8°C
NB-MSC Coating Solution: 2-8°C
Physiologix XF SR: ≤-70°C
Shelf Life 90 days – analysis ongoing
Research Category MSC research

Documents and Downloads

Take a closer look at our helpful product information to learn how our cell culture media products and services can support and accelerate your groundbreaking research.

NB-MSC™ Product Note

View more details about NB-MSC, the customizable mesenchymal stem cell media formula.

NB-MSC™ Media Performance White Paper

See how NB-MSC performs compared to the gold standard DMEM + 10% FBS.
Instructions for Use
Review the NB-MSC instructions for use to ensure proper use.

Need Help?

Get in touch with our customer support team for any questions you may have.

Visit NB-Lux™

See all available media products and customize according to your requirements.

Formulation Services

Leverage our experts to develop or optimize your media.

FAQs

Have a question? We’re here to help. If you don’t see your question, visit our FAQs page or contact us.
What is NB-MSC™ Serum-Free Medium?

NB-MSC is a serum-free and xeno-free media designed specifically to support proliferation in mesenchymal stem cells (MSCs). NB-MSC is part of our QuickStart Media portfolio, where it can be supplied as an RUO sample for evaluation, then customized with modifications to components and packaging to meet your desired specifications.

How can I request a sample of NB-MSC™?

Contact our sales team to request a sample of the base formula for NB-MSC.

How can I customize and/or order NB-MSC™?

Contact our sales team. After reviewing your project needs, they can coordinate a meeting with our scientific experts to customize and optimize your MSC cell culture media or arrange for shipment of your order.

Why can’t I order or customize NB-MSC™ in NB-Lux™?

NB-MSC is a proprietary pre-optimized mesenchymal stem cell culture medium formula. Due to the nature of not being an off-the-shelf product, customers must schedule a meeting with our sales team to order or customize NB-MSC Culture Medium.

What cell types has NB-MSC™ been tested for?

NB-MSC has been tested to perform on mesenchymal stem cells (MSCs). NB-MSC was designed to specifically support proliferation of mesenchymal stem cells. Read our white paper to learn more about NB-MSCs performance.

How much does a customized NB-MSC™ medium cost?

Due to the custom nature of NB-MSC – customization of components, concentrations, packaging, testing, and quality – pricing is variable. Contact our sales team to request a quote.