NB-KUL™ DF Cryopreservation Media
NB-KUL DF is a high-performing xeno-free, DMSO-free, and chemically-defined QuickStart cryopreservation medium that is ready to use or customizable to meet your process needs. Configure components, concentrations, packaging, and quality.
The NB-KUL™ Advantage
Customizable
Start with our pre-optimized cryomedia formula or use it as a base to adjust to your process needs in terms of packaging configurations, quality, and overall composition.
Proven Performance
Tested for the cryopreservation of PBMCs, T cells, NK cells, MSCs, and HEK cells, NB-KUL DF delivers equivalent or superior performance to industry-leading cryopreservation media.
Transparent Process
DMSO Performance Without the DMSO
Many cryomedia options on the market today contain various amounts of DMSO. This can present significant challenges in cell and gene therapy development due to its cytotoxicity and potential impact on cells and patients. Furthermore, most formulations and packaging are locked and proprietary and do not provide opportunities for customization, complicating downstream processes. Part of our QuickStart Media platform, NB-KUL DF is a customizable, chemically-defined, and DMSO-free cryopreservation media that offers users convenience and cost advantages.
Maintain Viability Equivalent to DMSO-Containing Media
NB-KUL DF maintains cell viability at equivalent levels as CryoStor® CS2 and CryoStor® CS5, and is significantly better than both Cell-Vive™ CD DMSO-Free and CryoStor® CSB freeze media after a freeze-thaw cycle with PBMCs. Cell recovery and expansion (assessed but not shown) are also comparable.
Recover More Cells Post Thaw
NB-KUL DF supports superior recovery post thaw in a T cell model when compared to CryoStor® CS5 and CryoStor® CSB. Viability and population doubling are also comparable with T cells.
Promote Robust Proliferation Post Thaw
An analysis of cell expansion using MSC demonstrates significantly better growth post-thaw when cells were stored in NB-KUL DF compared to CryoStor® CSB. When compared to CryoStor® CS5, NB-KUL DF performs equivalently demonstrating that viability and health of stored cells is achieved in the absence of DMSO. Post-thaw viability and cell count in MSCs (assessed but not shown) show comparable results.
How QuickStart Media™ Works
As part of our QuickStart Media platform, NB-KUL DF can be ordered as an RUO sample, tested in your process, and then customized and packaged according to your requirements. This service will help streamline the formulation development process of in-demand media products and truly expedite your cell therapy projects.
STEP 1
Order RUO Sample
Complete our request form to order an RUO sample of NB-KUL DF.
STEP 2
Test RUO Sample
Test the performance of NB-KUL DF on your project and review the data.
STEP 3
Customize Your Media
NB-KUL™ DMSO-Free Cryopreservation Media
NB-KUL™ DF RUO Sample
Catalog number: 2103
100 mL
$299
RUO sample. NB-KUL DF is a DMSO-free, chemically-defined, xeno-free cryopreservation medium. For research use only.
NB-KUL™ DF Custom
Volume: Custom
Inquire for price
Customize NB-KUL DF to meet your requirements. NB-KUL DF is a DMSO-free, chemically-defined, xeno-free cryopreservation medium.
Specifications
| Type | Cryopreservation medium |
| Classification | Xeno-free, serum-free |
| Quality | RUO |
| Sterility | Sterile filtered through a 0.2 micron filter |
| Format | Liquid |
| Volume | 100 mL |
| DMSO Level | None |
| pH | 6 to 8 |
| Shipping Conditions | 2-8°C |
| Storage Temperature | 2-8°C |
| Research Category | T cell research, stem cell research, NK cell research, gene therapy, viral vector manufacturing |
Documents and Downloads
Take a closer look at our helpful product information to learn how our cell culture media products and services can support and accelerate your groundbreaking research.
NB-KUL™ DF Product Note
View more details about NB-KUL DF, the customizable DMSO-free cryopreservation media formula.
NB-KUL™ DF Cryopreservation Media Performance White Paper
See how NB-KUL DF performs compared to competing cryopreservation media.
Need Help?
Get in touch with our customer support team for any questions you may have.
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FAQs
What is cryopreservation media?
Cryopreservation is the process of preserving cells, tissues, organs, or other biological materials at very low temperatures, suspending all biological activities and metabolic processes while avoiding damage caused by the formation of ice crystals during freezing. To achieve this, a cryopreservation media is needed. Cryopreservation media is a freeze media that consists of cryoprotective agents in combination with salts, sugars, amino acids, proteins, and other compounds to maintain proper pH and osmotic balance.
How can I request a sample of NB-KUL™?
How can I customize and/or order NB-KUL™ DF?
Contact our sales team. After reviewing your project needs, they can coordinate a meeting with our scientific experts to customize and optimize your cryomedia or arrange for shipment of your order.
Why can’t I order or customize NB-KUL™ DF in NB-Lux™?
NB-KUL is a proprietary pre-optimized cryopreservation medium formula. Due to the nature of not being an off-the-shelf product, customers must schedule a meeting with our sales team to order or customize an NB-KUL product.
What cell types has NB-KUL™ DF been tested for?
Currently, NB-KUL DF has been tested for the preservation of PBMCs, T cells, NK cells, MSCs, and HEK cells. NB-KUL DF delivers equivalent or superior performance to industry-leading cryopreservation media.
How much does NB-KUL™ DF cost?
Due to the custom nature of NB-KUL DF – customization of components, concentrations, packaging, testing, and quality – pricing is variable. Contact our sales team to request a quote.