Physiologix XF SR

PhysiologixTM XF SR

Human Growth Factor Concentrate

A cGMP, xeno-free media supplement made for stem cells and T cells that replaces FBS or human serum.

PhysiologixTM XF SR

Many "serum-free" supplements and complete media don't disclose their origin or contents.

Our serum replacement is extracted from transfusion-grade blood products and comes with a full certificate of analysis.

Physiologix XF SR Supplemented @ 2%
Step 1
PhysiologixTM XF SR
Supplemented @ 2%
Preferred Basal Media (e.g. RPMI 1640)
Step 2
Preferred Basal Media
(e.g. X-VIVO 15)
Cell Culture w/ Superior Proliferation
Step 3
Cell Culture w/
Superior Proliferation

ISSCR 2019 Poster

Authors: Saba Ghassemi, David Heo, Francisco J. Martinez-Becerra, John Leferovich , Sarah Richman, Alyssa M. Master, and Roddy S. O’Connor

Nucleus Biologics - ISSCR 2019 Poster

Adoptive immunotherapy involves patient T cell expansion in optimal conditions for the purpose of re-infusing their progeny as therapy. This calls for the generation and characterization of novel media supplements and formulations to boost the progress of the cell therapy market by providing physiologically relevant protein sources in concentrations suitable for biological activity. Physiologix™ XF Human Growth Factor Concentrate (hGFC) is a cGMP, xeno-free serum replacement that replaces supplements such as fetal bovine serum (FBS) or human serum (HS). In this study, T cell media such as OpTmizer and X-VIVO 15 were supplemented with 5% human serum or 2% Physiologix™ XF. In addition, RPMI 1640 with 10% FBS was compared due to its prevalent use in non-clinical settings. T cells from three healthy donors were activated with CD3/CD28 Dynabeads and then cultured for 14 days in varying media formulations. Proliferative capacity was assessed with flow cytometry. Physiologix™ XF showed increased potency relative to human serum when used along X-VIVO 15 or OpTmizer media. Differentiation status was assessed using CCR7 and CD45RO to distinguish T cell subpopulations. We detected an increase in naïve and central memory populations when cells were cultured in Physiologix™ XF compared to human serum. These phenotypes are highly relevant for the outcome of patients undergoing CAR-T cell therapy. To further define the advantage of our supplement in generating therapy ready products, we compared the transduction efficiency of a lentiviral-GFP in OpTmizer, X-VIVO 15 or RPMI supplemented with 2% Physiologix™ XF or 5% HS at different multiplicities of infection (MOIs). A higher transduction efficiency was consistently observed when using Physiologix™ XF at a high MOI of 4 down to a MOI of <1. These results were also observed for anti-GD-2 and anti-EGFR virus. This increased transduction efficiency and preservation of T cell subpopulations can translate into a higher number of transfusable CAR+ cells with relevant phenotypes which allow for reduced overall costs and better clinical outcomes. The ability of anti-GD-2 CAR-T cells expanded in medium supplemented with Physiologix™ XF to directly kill target cells and control tumor burden in an aggressive leukemia xenograft model is discussed.

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Our three product pillars to address your Xeno-Free needs

Streamlined Regulatory Compliance - cGMP

Streamlined Regulatory Compliance

  • Completely Xeno-Free
  • Processed under cGMP conditions
  • Transfusion-grade raw material
Lower Media Costs with Superior Yield

Lower Media Costs with Superior Yield

  • Superior proliferation in both T cells and stem cells
  • Lower acquisition cost and cost per target cell count
Consistent and
Concentrated

Consistent and
Concentrated

  • 2% recommended concentration in basal media (FBS requires 10%)
  • Documented consistency across lots