In celebration of the holidays, we take a look back at some of the most exciting events and achievements of the year…and a look forward to what the New Year will bring. Science enthusiasts everywhere, your attention please! Today we thank the hardworking team at...
Physiologix™ XF SR
HUMAN GROWTH FACTOR CONCENTRATE
A cGMP, xeno-free media supplement made for stem cells and T cells that replaces FBS or human serum.
$990 / 100 mL (Part Number: 322)
See the latest research! We, along with our collaborators at the University of Pennsylvania, were recently published in the journal Molecular Therapy – Methods & Clinical Development. Physiologix demonstrated superior solid tumor cytotoxicity compared to cells grown in human serum. Read the full article here.
Physiologix™ XF SR
Many “serum-free” supplements and complete media don’t disclose their origin or contents.
Our serum replacement is extracted from transfusion-grade blood products and comes with a full certificate of analysis.
New state-of-the-art cleanrooms will be the centerpiece of the already thriving small business, as the company seeks to expand their GMP-compliant manufacturing capabilities. Nucleus Biologics’ newly expanded facility is officially open for business! A festive...
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Improved T Cell Proliferation and Transduction Efficiency Ex Vivo for CAR-T therapy in a Xeno- and Serum-Free Medium.
NB-ROC T Cell Poster
Authors: Asma Ayari, Francisco J Martinez-Becerra, Yinan Tu, Yinan Lu, and Roddy O’Connor
Efforts need to be made to improve and develop a completely defined and xeno-free cell culture medium. Accordingly, components of animal origin used in the culture of T cells should be replaced with relevant protein sources and concentrations. Using a combination of metabolomics and process optimization, Nucleus Biologics has identified a new formulation named NB-ROC.
In this study, we tested NB-ROC on T cells isolated from the blood of three donors using standard protocols. Our data show that NB-ROC allowed cells to grow in higher numbers compared to OpTmizer™ (Fisher Scientific). This increase in proliferation did not compromise early memory phenotypes. Additionally, transduction using a GFP lentivirus was ~40% higher when cells were grown and activated in NB-ROC, which results in optimization of virus usage and reduction of manufacturing costs.
CAR-T Media Development: Novel Formulations Focused on Improved Transduction Efficiency and Preservation of Relevant T Cell Subpopulations
Authors: Saba Ghassemi, David Heo, Francisco J. Martinez-Becerra, John Leferovich , Sarah Richman, Alyssa M. Master, and Roddy S. O’Connor
Adoptive immunotherapy involves patient T cell expansion in optimal conditions for the purpose of re-infusing their progeny as therapy. This calls for the generation and characterization of novel media supplements and formulations to boost the progress of the cell therapy market by providing physiologically relevant protein sources in concentrations suitable for biological activity. Physiologix™ XF Human Growth Factor Concentrate is a cGMP, xeno-free serum replacement that replaces supplements such as fetal bovine serum or human serum. Review this poster to view the study results of optimizing T cell media with Physisologix.
Our three product pillars to address your Xeno-Free needs
Lower Media Costs with Superior Yield
- Superior proliferation in both T cells and stem cells
- Lower acquisition cost and cost per target cell count
- 2% recommended concentration in basal media (FBS requires 10%)
- Documented consistency across lots