HEKima™ Adherent: Serum-Free Cell Culture Media Formula Specifically Developed for Adherent HEK-293 Culture Without Cell Weaning
Introduction
Improving production efficiencies is essential to meet the growing demand for lentiviral and AAV vectors which are used in cell and gene therapy manufacturing. HEK-293 cells play a crucial role in this process. HEK-293 cells are available in multiple variants, each tailored through specific manipulations either to enhance protein or virus production. This white paper summarizes the performance of HEKima Adherent, a serum-free and xeno-free cell culture medium to specifically expand adherent HEK cells and boost their protein expression without the need for a gradual adaptation to serum-free media.
In this white paper, we will share HEKima Adherent performance with different HEK cell variants compared to the gold standard (DMEM + 10% FBS) for adherent cells. See the data for yourself!
- Evaluation of cell proliferation and morphology
- Assessing effects of subcultures on confluency
- Quantification of protein expression after transfection with lentivirus containing GFP
- Evaluating growth of HEK variants in HEKima Adherent media