NB-KUL™ DF: A Chemically-Defined, DMSO-Free Cryopreservation Medium Specifically Designed for Cell and Gene Therapies
Introduction
Cryopreservation is essential for the success of cell and gene therapy workflows, ensuring that cells maintain their functionality and viability through freezing and thawing. Optimized cryopreservation solutions are crucial for preserving cell integrity and enhancing therapeutic efficacy. DMSO is often used in these solutions due to its protective properties; however, its use presents challenges such as regulatory hurdles, potential toxicity, and the need for post-thaw removal, complicating the process and increasing costs.
This white paper explores the performance of NB-KUL DF, a chemically-defined and DMSO-free cryopreservation medium designed for various human cell types. We benchmarked its effectiveness against CryoStor® CS2, CryoStor® CS5, CryoStor® CSB, and Cell-Vive™ CD DMSO-Free, focusing on critical post-thaw metrics like cell count, viability, and recovery during cell expansion. Our findings highlight the potential for DMSO-free solutions to improve operational efficiency and patient safety in cell therapies.
See the data for yourself!
- Performance on PBMCs
- Performance on T Cells
- Performance on NK Cells
- Performance on MSCs