T Cell Media Guide: A Comprehensive Guide to Key Components

24 Jun 2021

The surge of cellular T cell-based immunotherapies has prompted more and more researchers to take an interest in these specialized immune cells. Whether you are brand new to the cell therapy field or an experienced immunologist, learning to work with a new cell type can often lead you to feel overwhelmed. In this comprehensive guide we will explain the ins and outs of culturing T cells, so you can spend less time searching for answers to your T cell questions and more time making discoveries.

Explore the key players in adaptive immunity, the components of T cell media and what they do, and why you should be looking for alternatives to your FBS and HS for your adoptive T cell therapies.


What you can expect to find in the T Cell Media Guide

  • T Cells: Key Players in Adaptive Immunity
  • Adoptive T Cell Therapy (CAR-T)
  • CAR-T Cell Media Requirements
  • The Basics of T Cell Culture
  • T Cell Media
  • T Cell Media Components: What are they & what do they do?
T Cell Components Discussed
Buffering Systems Protein Amino Acids
Carbohydrates Lipids Inorganic Salts
Trace Elements Vitamins β-Mercaptoethanol
Interleukins Antibiotics


Want To Read The Full Guide?

Download as PDF

T-Cell Media A Comprehensive Guide to Key Components

Share this article

Alyssa Master, Ph.D.
Dr. Alyssa Master is the VP of Sales Enablement and Customer Experience at Nucleus Biologics. She is a subject matter expert on cell culture media solutions, primarily on the development of custom cell culture media. Dr. Master has ten years of laboratory research experience, including five years as a lab manager prior to joining Nucleus Biologics. As a biomaterials engineer, her area of expertise is in the field of nanoparticle-mediated drug delivery, particularly for the delivery of cancer therapeutics. During her numerous fellowships, Dr. Master developed a novel targeted drug delivery platform for intravenous delivery of photodynamic therapy agents. Dr. Master earned her Ph.D. in Biomedical Engineering from Case Western Reserve University.